Western blot analysis of extracts of various cell lines, using RNF4 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

RNF4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C747247-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: RNF4 antibody LS-C747247 is an unconjugated rabbit polyclonal antibody to RNF4 from human. It is reactive with human, mouse and rat. Validated for WB.
Application: IHC, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Ring finger protein 4 | Protein SNURF | SLX5 | E3 ubiquitin ligase RNF4 | RES4-26 | RNF4
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: P78317
NCBI GenBank: NM_002938 | NP_002929.1
SwissProt: P78317

Target

Target: Human RNF4
Antigen Species: Human
Gene Name: RNF4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1). MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)