Western blot analysis of extracts of various cell lines, using RNF181 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

RNF181 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C749409-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: RNF181 antibody LS-C749409 is an unconjugated rabbit polyclonal antibody to RNF181 from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: LOC51255 | Ring finger protein 181 | HSPC238 | RNF181
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9P0P0
NCBI GenBank: NM_016494 | NP_057578.1
SwissProt: Q9P0P0

Target

Target: Human RNF181
Antigen Species: Human
Gene Name: RNF181

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1). MASYFDEHDCEPSDPEQETRTNMLLELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDDDTYEEHRRDKARKQQQQHRLENLHGAMYT
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)