Western blot of human GRAIL in human spleen lysate in the 1) absence and2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using antibody at1 ug/ml.
RNF128 / GRAIL Antibody (aa227-253)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Bovine, Dog, Horse, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C47370-100
Catalog Number: LS-C47370
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- Description
- GRAIL antibody LS-C47370 is an unconjugated rabbit polyclonal antibody to GRAIL (RNF128) (aa227-253) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Bov, Can, Hr, Hu, Ms, NHP, Por, Rb, Rt
- Predicted Reactivity
- Mammal (Other)
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GRAIL | RING finger protein 128 | RNF128
- Recommended Dilution: IHC-P
- 5 - 10 µg/ml
- Recommended Dilution: WB
- 0.5 - 2 µg/ml
- UniProt Number
- Q8TEB7
- NCBI GenBank
- NM_024539 | NP_078815.3
- SwissProt
- Q8TEB7
Target
- Target
- Human RNF128 / GRAIL
- Antigen Species
- Human
- Epitope
- A synthetic peptide corresponding to amino acids (IFYSARRLRNARAQSRKQRQLKADAKK) portion of amino acids 227-253 of human GRAIL was used as immunogen, GenBank no. ref|NP_919445.1|.
- Gene Name
- RNF128
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids (IFYSARRLRNARAQSRKQRQLKADAKK) portion of amino acids 227-253of human GRAIL, GenBank no. ref|NP_919445.1|. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Bat (96%); Opossum (93%); Platypus (89%); Turkey, Chicken (85%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS