Rhodopsin / RHO Antibody (clone Rho 1D4)

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Antibody:	Rabbit Recombinant Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Radioimmunoassay, Western Blot
Reactivity:	Amphibian (Other), Bovine, Human, Mouse, Rat, Zebrafish
Format:	Liquid

$551.00each

Catalog Number: LS-C821425-0.2

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Description: This Rhodopsin / RHO antibody is an unconjugated Rabbit/Human chimeric recombinant monoclonal antibody. Validated for ELISA, IF, IHC, IP, RIA, and WB.
Application: ELISA, IF, IHC, IP, RIA, WB
Reactivity: Amph, Bov, Hu, Ms, Rt, Zf
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names: CSNBAD1 | OPN2 | Rhodopsin | RP4 | Opsin 2, rod pigment | Opsin-2 | RHO
UniProt Number: P08100
NCBI GenBank: NM_000539 | NP_000530.1
SwissProt: P08100

Target: Bovine Rhodopsin / RHO
Antigen Species: Bovine
Epitope: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Gene Name: RHO

Storage Conditions: Store undiluted at 4°C for up to 3 months. Aliquot and store at -20°C for long term storage.
Recommended Storage Buffer: PBS

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