Western blot analysis of extracts of various cell lines, using RHOB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

RHOB Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C747507-20

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Overview

Description: RHOB antibody LS-C747507 is an unconjugated rabbit polyclonal antibody to RHOB from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Aplysia RAS-related homolog 6 | ARH6 | H6 | MST081 | Oncogene RHO H6 | RHOH6 | Ras homolog family member B | ARHB | Transforming protein rhob | MSTP081 | Rho cDNA clone 6 | RHOB
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P62745
NCBI GenBank: NM_004040 | NP_004031.1
SwissProt: P62745

Target

Target: Human RHOB
Antigen Species: Human
Gene Name: RHOB

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human RHOB (NP_004031.1). MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQKRYGSQNGCINCCKVL
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)