anti-NFkB antibody. Affinity purified Anti-NFKB p65 (Rel A) pS276 Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 600-401-264 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.
RELA / NFKB p65 Antibody (phospho-Ser276)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C154220-100
Catalog Number: LS-C154220
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- Description
- NFKB p65 antibody LS-C154220 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer276). Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
- Recommended Dilution: ELISA
- 1:5000 - 1:25000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q04206
- NCBI GenBank
- NM_021975 | NP_068810.3
- SwissProt
- Q04206
Target
- Target
- Human RELA / NFKB p65
- Antigen Species
- Human
- Epitope
- This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.
- Gene Name
- RELA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002;169:6490-7. PubMed: 12444159