Western blot analysis of extracts of various cell lines, using RELA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
RELA / NFKB p65 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$305.00each
Catalog Number: LS-C497320-20
Catalog Number: LS-C497320
For bulk orders or custom formulations:
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- Description
- NFKB p65 antibody LS-C497320 is an unconjugated mouse monoclonal antibody to NFKB p65 (RELA) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.
- Application
- IF, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:20 - 1:50
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q04206
- NCBI GenBank
- NM_021975 | NP_068810.3
- SwissProt
- Q04206
Target
- Target
- Human RELA / NFKB p65
- Antigen Species
- Human
- Gene Name
- RELA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant protein of human RELA
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS