Western blot analysis of extracts of various cell lines, using RB1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
RB1 / Retinoblastoma / RB Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C746962-20
Catalog Number: LS-C746962
For bulk orders or custom formulations:
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- Description
- RB antibody LS-C746962 is an unconjugated rabbit polyclonal antibody to RB (RB1 / Retinoblastoma) from human. It is reactive with human and mouse. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- p105-Rb | Osteosarcoma | PRb | RB1 | Retinoblastoma | Retinoblastoma 1 | OSRC | RB | Pp110
- Recommended Dilution: IHC
- 1:20 - 1:100
- Recommended Dilution: WB
- 1:200 - 1:500
- UniProt Number
- P06400
- NCBI GenBank
- NM_000321 | NP_000312.2
- SwissProt
- P06400
Target
- Target
- Human RB1 / Retinoblastoma / RB
- Antigen Species
- Human
- Gene Name
- RB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide of human RB1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS