LSBio
Rat Anti-Rabbit IgG Secondary Antibody (HRP)

Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Rat Anti-Rabbit IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Rat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$429.00each
Catalog Number: LS-C60921-1

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Overview

Description
Rabbit IgG antibody LS-C60921 is an HRP-conjugated rat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:10000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Goat, Human and Mouse Serum Proteins.

Antibody

Host Species
Rat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Pleiotropic platelet defects in mice with disrupted FOG1-NuRD interaction. Wang Y, Meng R, Hayes V, Fuentes R, Yu X, Abrams CS, Heijnen HF, Blobel GA, Marks MS, Poncz M. Blood. Dec;118:6183-91. PubMed: 21989988 PMC: PMC3234671

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