Western blot analysis of extracts of various cell lines, using RAP1GAP antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

RAP1GAP Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human
Format:	Liquid

$281.00each

Catalog Number: LS-C497248-20

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Overview

Description: RAP1GAP antibody LS-C497248 is an unconjugated rabbit polyclonal antibody to human RAP1GAP. Validated for WB.
Application: WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: KIAA0474 | RAP1GAP | RAP1 GTPase activating protein | RAP1GA1 | RAP1GAP1 | RAP1GAPII | RAPGAP
Recommended Dilution: WB: 1:1000 - 1:2000
UniProt Number: P47736
SwissProt: P47736

Target

Target: Human RAP1GAP
Antigen Species: Human
Gene Name: RAP1GAP

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human RAP1GAP (NP_001139130.1). AMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)