LSBio
Rabbit Anti-Mouse IgG1 Secondary Antibody (HRP)

Western blot of Peroxidase conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.

Rabbit Anti-Mouse IgG1 Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG1 antibodies →
$414.00each
Catalog Number: LS-C60782-1

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Overview

Description
Mouse IgG1 antibody LS-C60782 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:200000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:2000 - 1:20000

Target

Target
Mouse IgG1
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is 1%.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG1 heavy chain

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.