Anti-Mouse IgG Secondary Antibody
Catalog Number: LS-C751594-1
1 mg / $265.00
Antibody: Mouse Monoclonal
Format: Lyophilized
ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Rabbit Anti-Mouse IgG Secondary Antibody (Atto 594)
Secondary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Other, Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$300.00each
Catalog Number: LS-C745587-100
Catalog Number: LS-C745587
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Mouse IgG antibody LS-C745587 is an Atto 594-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
- Application
- IF, Other, WB
- Reactivity
- Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: IF/ICC
- 1:5000
- Recommended Dilution: Other
- FLISA: 1:20000
- Recommended Dilution: WB
- 1:10000
Target
- Target
- Mouse IgG
- Target Heavy Chain
- IgG
- Antigen Species
- Ms
- Epitope
- This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
- Immunogen Type
- Native
Format
- Conjugate/Label
- Atto 594
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
