LSBio
Rabbit Anti-Mouse IgG Secondary Antibody (Atto 425)

ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT].

Rabbit Anti-Mouse IgG Secondary Antibody (Atto 425)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG antibodies →
$300.00each
Catalog Number: LS-C745584-100

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Overview

Description
Mouse IgG antibody LS-C745584 is an Atto 425-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
Application
IF, Other, WB
Reactivity
Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: IF/ICC
1:5000
Recommended Dilution: Other
FLISA: 1:20000
Recommended Dilution: WB
1:10000

Target

Target
Mouse IgG
Target Heavy Chain
IgG
Antigen Species
Ms
Epitope
This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Immunogen Type
Native

Format

Conjugate/Label
Atto 425
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.