LSBio
Rabbit Anti-Human IgG Secondary Antibody (Biotin)

Western Blot of Biotin Conjugated Rabbit Anti-Human IgG Pre-Adsorbed Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IgG Antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): None.

Rabbit Anti-Human IgG Secondary Antibody (Biotin)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
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$393.00each
Catalog Number: LS-C60623-2

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Overview

Description
Human IgG antibody LS-C60623 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IB, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Thimerosal
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.01 M Sodium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.