LSBio
Rabbit Anti-Human IgG Secondary Antibody (AP)

Dot Blot of Rabbit Anti-Human IgG gamma chain Alkaline Phosphatase Conjugated Antibody. Lane 1: 100ng hu IgG. Lane 2: 33.3ng hu IgG. Lane 3: 11.1ng hu IgG. Lane 4: 3.70ng hu IgG. Lane 5: 1.23ng hu IgG. Secondary Antibody: Rt-a-Hu IgG Alk Phos at 1µg/mL.

Rabbit Anti-Human IgG Secondary Antibody (AP)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
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$439.00each
Catalog Number: LS-C60614-1

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Overview

Description
Human IgG antibody LS-C60614 is an AP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:10000 - 1:20000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Human IgG
Target Heavy Chain
IgG
Antigen Species
Hu
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG gamma heavy chain.
Immunogen Type
Native

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.05% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.