LSBio
Pyruvate Kinase Antibody (Lyophilized)

Western Blot of Goat Anti-Pyruvate Kinase Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker Primary Antibody: Goat Anti-Pyruvate Kinase at 1:50 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT. Expect: ~57kDa.

Pyruvate Kinase Antibody (Lyophilized)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
See all Rabbit Pyruvate Kinase antibodies →
$579.00each
Catalog Number: LS-C209624-100

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Overview

Description
Pyruvate Kinase antibody LS-C209624 is an unconjugated goat polyclonal antibody to rabbit Pyruvate Kinase. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: IHC
1:500 - 1:2000
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Rabbit Pyruvate Kinase
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Pyruvate Kinase (Rabbit Muscle).

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.