LSBio
Pyruvate Kinase Antibody (Lyophilized, Biotin)

Western Blot of Goat Anti-Pyruvate Kinase Biotin Conjugate. Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: 50ng of Pyruvate Kinase. Primary Antibody: Goat Anti-Pyruvate Kinase Biotin at 1ug/mL o/n @ 4C. Secondary Antibody: Streptavidin-HRP: Sat 1:40,000 for 30 min at RT.

Pyruvate Kinase Antibody (Lyophilized, Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Rabbit
Format:Lyophilized
See all Rabbit Pyruvate Kinase antibodies →
$564.00each
Catalog Number: LS-C209693-100

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Overview

Description
Pyruvate Kinase antibody LS-C209693 is a biotin-conjugated goat polyclonal antibody to rabbit Pyruvate Kinase. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: WB
1:200 - 1:1000

Target

Target
Rabbit Pyruvate Kinase
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Pyruvate Kinase [Rabbit Muscle].

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.