Western Blot of Goat Anti-Pyruvate Kinase Biotin Conjugate. Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: 50ng of Pyruvate Kinase. Primary Antibody: Goat Anti-Pyruvate Kinase Biotin at 1ug/mL o/n @ 4C. Secondary Antibody: Streptavidin-HRP: Sat 1:40,000 for 30 min at RT.

Pyruvate Kinase Antibody (Liquid, Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Goat Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Rabbit
Format:	Liquid

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$311.00each

Catalog Number: LS-C744814-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Pyruvate Kinase antibody LS-C744814 is a biotin-conjugated goat polyclonal antibody to rabbit Pyruvate Kinase. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Rb
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: WB: 1:200 - 1:1000

Target

Target: Rabbit Pyruvate Kinase
Antigen Species: Rb
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

Antibody

Host Species: Gt
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Pyruvate Kinase [Rabbit Muscle].

Format

Conjugate/Label: Biotin
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: IEX

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl