Western blot analysis of extracts of various cell lines, using PYCARD antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PYCARD / ASC / TMS1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C331318-20
Catalog Number: LS-C331318
For bulk orders or custom formulations:
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- Description
- TMS1 antibody LS-C331318 is an unconjugated rabbit polyclonal antibody to TMS1 (PYCARD / ASC) from human. It is reactive with human and mouse. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CARD5 | HASC | PYCARD | ASC | TMS | TMS-1 | PYD and CARD domain containing | TMS1
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9ULZ3
- NCBI GenBank
- NM_013258 | NP_037390.2
- SwissProt
- Q9ULZ3
Target
- Target
- Human PYCARD / ASC / TMS1
- Antigen Species
- Human
- Epitope
- Human PYCARD / ASC
- Gene Name
- PYCARD
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 50-195 of human PYCARD (NP_037390.2). LDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS