Fluorescent image of HeLa cell stained with SHP2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SHP2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SHP2 immunoreactivity is localized to Nucleolus and Cytoplasm significantly and Nucleus weakly.
PTPN11 / SHP-2 / NS1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C101075-400
Catalog Number: LS-C101075
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- Description
- NS1 antibody LS-C101075 is an unconjugated rabbit polyclonal antibody to human NS1 (PTPN11 / SHP-2). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Bptp-3 | BPTP3 | CFC | Csw | Corkscrew csw | Gene corkscrew | Phosphatase corkscrew | PTP-2C | SHP2 | PTP-1D | PTP2C | PTPN11 | SHP-2 | SHPTP2 | Noonan syndrome 1 | SH-PTP2 | SH-PTP3
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q06124
- NCBI GenBank
- NM_002834 | NP_002825.3
- SwissProt
- Q06124
Target
- Target
- Human PTPN11 / SHP-2 / NS1
- Antigen Species
- Human
- Epitope
- This SHP2 antibody is generated from rabbits immunized with a recombinant protein of partial human SHP2.
- Gene Name
- PTPN11
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS