Western blot analysis of extracts of various cell lines, using PSMB1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

PSMB1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C331217-20

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Overview

Description: PSMB1 antibody LS-C331217 is an unconjugated rabbit polyclonal antibody to PSMB1 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Application: IF, IHC, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Macropain subunit C5 | HC5 | PMSB1 | Proteasome gamma chain | Proteasome subunit beta 1 | Proteasome beta 1 subunit | Proteasome component C5 | PSMB1 | Lmpc5 | Proteasome subunit beta type-1 | Proteasome subunit HC5 | PSC5
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: IHC: 1:50 - 1:200
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P20618
NCBI GenBank: NM_002793 | NP_002784.1
SwissProt: P20618

Target

Target: Human PSMB1
Antigen Species: Human
Epitope: Human PSMB1
Gene Name: PSMB1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1). RFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKD
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)