Dot Blot results of rabbit Anti-Trypsin Peroxidase Conjugated. Antigen: Trypsin. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Trypsin HRP 10µg/mL for 1hr at RT. Secondary Antibody: none. Imaging System ChemiDoc, Filter used: Chemi.
PRSS1 / Trypsin Antibody (Liquid, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745032-25
Catalog Number: LS-C745032
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Trypsin antibody LS-C745032 is an HRP-conjugated rabbit polyclonal antibody to bovine Trypsin (PRSS1). Validated for DB, ELISA, IHC and WB.
- Application
- DB, ELISA, IHC, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Beta-trypsin | Cationic trypsinogen | Digestive zymogen | HPC | Hereditary pancreatitis | TRP1 | TRY4 | Trypsin | Trypsinogen i | TRYP1 | Trypsinogen | TRY1 | Trypsinogen 1 | Nonfunctional trypsin 1 | Protease serine 1 | PRSS1 | Serine protease 1 | Trypsin I | Trypsin-1 | Trypsinogen A
- Recommended Dilution: WB
- 1:100 - 1:500
- UniProt Number
- P07477
- NCBI GenBank
- NM_002769 | NP_002760.1
- SwissProt
- P07477
Target
- Target
- Bovine PRSS1 / Trypsin
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources is unknown.
- Gene Name
- PRSS1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl