LSBio
Protein Kinase A, Substrate, Phosphorylated Antibody (phospho-Ser/Thr)

Protein Kinase A, Substrate, Phosphorylated Antibody (phospho-Ser/Thr)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Format:Liquid
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$703.00each
Catalog Number: LS-C75168-100

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Overview

Description
Protein Kinase A, Substrate, Phosphorylated antibody LS-C75168 is an unconjugated rabbit polyclonal antibody to Protein Kinase A, Substrate, Phosphorylated (pSer/Thr). Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IHC-P, IP, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:500
Recommended Dilution: IHC-P
1:200
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:1000

Target

Target
Protein Kinase A, Substrate, Phosphorylated
Epitope
Detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position. It is a useful tool in identifying new substrates of AGC family kinases, including PKA and PKC. It does not recognize the non-phosphorylated PKA substrate motif.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Synthetic phospho- PKA substrate peptide.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
Volume
100 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.