Anti-GSK3A Antibody - Western Blot. Western blot of Affinity Purified anti-GSK3A antibody shows detection of a 52 kD band corresponding to human GSK3A present in ~35 ug of HEK293 whole cell lysate (lane 1). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO in PBS. Primary antibody was used at a 1:1000 dilution in blocking buffer and reacted overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.