Staining HuvEc cells treated with serum 20% 30' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PRKDC / DNA-PKcs Antibody (phospho-Thr2647)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C801125-100
Catalog Number: LS-C801125
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- DNA-PKcs antibody LS-C801125 is an unconjugated rabbit polyclonal antibody to human DNA-PKcs (PRKDC) (pThr2647). Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DNA-PK | DNAPK | DNPK1 | HYRC | HYRC1 | p350 | PRKDC | Scid | XRCC7 | DNA-PK catalytic subunit | DNA-PKcs | p460 | DNA-dependent protein kinase catalytic subunit
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P78527
- NCBI GenBank
- NM_006904 | NP_008835.5
- SwissProt
- P78527
Target
- Target
- Human PRKDC / DNA-PKcs
- Antigen Species
- Human
- Epitope
- Phospho-DNA-PK (Thr2647) Antibody detects endogenous levels of DNA-PK only when phosphorylated at Threonine 2647.
- Gene Name
- PRKDC
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human DNA-PK around the phosphorylation site of Threonine 2647.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS