Western blot analysis of Mouse Brain showing detection of 78 kDa PRKCG protein using Rabbit Anti-PRKCG Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PRKCG Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 78 kDa. Other Band(s): 62 kDa, 40 kDa.
PRKCG / PKC-Gamma Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775009-100
Catalog Number: LS-C775009
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- Description
- PKC-Gamma antibody LS-C775009 is an unconjugated rabbit polyclonal antibody to PKC-Gamma (PRKCG) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- PKC-gamma | PRKC-gamma | Protein kinase C gamma | PRKCG | Protein kinase C gamma type | PKC Gamma | PKCC | PKCG | Pkcgamma | Protein kinase C, gamma | SCA14
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P05129
- NCBI GenBank
- NM_002739 | NP_002730.1
- SwissProt
- P05129
Target
- Target
- Human PRKCG / PKC-Gamma
- Antigen Species
- Human
- Epitope
- Detects 62 kDa, 40 kDa. 62 kDa band is isoform 2, band at 40 kDa is a degradation product.
- Gene Name
- PRKCG
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Human PRKCG (aa. 150-250)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS