Western blot analysis of extracts of U-937 cells, using Phospho-PRKCD-T505 antibody at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 60s.
PRKCD / PKC-Delta Antibody (phospho-Thr505)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750583-20
Catalog Number: LS-C750583
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- Description
- PKC-Delta antibody LS-C750583 is an unconjugated rabbit polyclonal antibody to human PKC-Delta (PRKCD) (pThr505). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MAY1 | NPKC-delta | PKCD | PRKCD | Protein kinase C delta type | Protein kinase C delta VIII | Protein kinase C, delta | Pkcdelta | PKC delta | Protein kinase c delta | Tyrosine-protein kinase PRKCD
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q05655
- NCBI GenBank
- NM_006254 | NP_006245.2
- SwissProt
- Q05655
Target
- Target
- Human PRKCD / PKC-Delta
- Antigen Species
- Human
- Gene Name
- PRKCD
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS