Western blot analysis of extracts from COLO205 cells, using PKC a (Phospho-Tyr657) antibody.
PRKCA / PKC-Alpha Antibody (phospho-Tyr657)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822146-100
Catalog Number: LS-C822146
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- Description
- PKC-Alpha antibody LS-C822146 is an unconjugated rabbit polyclonal antibody to PKC-Alpha (PRKCA) (pTyr657) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | PRKCA | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P17252
- NCBI GenBank
- NM_002737 | NP_002728.1
- SwissProt
- P17252
Target
- Target
- Human PRKCA / PKC-Alpha
- Antigen Species
- Human
- Epitope
- PKC Alpha (Phospho-Tyr657) antibody detects endogenous levels of PKC Alpha only when phosphorylated at tyrosine 657. (Human: Tyr657; Mouse: Tyr657; Rat: Tyr657)
- Gene Name
- PRKCA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human PKC Alpha around the phosphorylation site of tyrosine 657 (F-S-YP-V-N).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl