Western blot analysis of extracts of various cell lines, using PRKCA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PRKCA / PKC-Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$305.00each
Catalog Number: LS-C497767-20
Catalog Number: LS-C497767
For bulk orders or custom formulations:
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- Description
- PKC-Alpha antibody LS-C497767 is an unconjugated rabbit monoclonal antibody to PKC-Alpha (PRKCA) from human. It is reactive with human, mouse and rat. Validated for Flow, IF, IHC, IP and WB.
- Application
- FC, IF, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | PRKCA | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:20 - 1:50
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P17252
- NCBI GenBank
- NM_002737 | NP_002728.1
- SwissProt
- P17252
Target
- Target
- Human PRKCA / PKC-Alpha
- Antigen Species
- Human
- Gene Name
- PRKCA
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
Immunogen
- Immunogen
- Recombinant protein of human PRKCA
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
Storage & Handling
- Recommended Storage Buffer
- PBS