Western Blot of rabbit anti-Retinoblastoma [Monomethyl Lys860] antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: MOLT-4 WCL. Load: 35 ug per lane. Primary antibody: Retinoblastoma antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 30 min at RT. Block: BlockOut 30 min at RT. Predicted/Observed size: expect 106 kDa for Retinoblastoma protein.

PRB1 Antibody (Me-Lys860)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$311.00each

Catalog Number: LS-C745429-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: PRB1 antibody LS-C745429 is an unconjugated rabbit polyclonal antibody to human PRB1 (Me-Lys860). Validated for DB, ELISA, IHC, IP and WB.
Application: DB, ELISA, IHC, IP, WB
Reactivity: Hu
Predicted Reactivity: Mouse, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Salivary proline-rich protein | Proline-rich peptide II-2 | Basic peptide IB-6 | Peptide P-H | PRB1M | PRB1L
Recommended Dilution: ELISA: 1:10000 - 1:50000
Recommended Dilution: WB: 1 µg/ml
NCBI GenBank: NM_005039 | NP_005030.2

Target

Target: Human PRB1
Antigen Species: Human
Epitope: This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.99 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl