Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PR / Progesterone Receptor Antibody (phospho-Ser400)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800968-100
Catalog Number: LS-C800968
For bulk orders or custom formulations:
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- Description
- Progesterone Receptor antibody LS-C800968 is an unconjugated rabbit polyclonal antibody to Progesterone Receptor (PR) (pSer400) from human. It is reactive with human and monkey. Validated for IF, Peptide-ELISA and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NR3C3 | PGR | PR | Progesterone receptor
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P06401
- NCBI GenBank
- AAS00096.1 | NM_000926
- SwissProt
- P06401
Target
- Target
- Human PR / Progesterone Receptor
- Antigen Species
- Human
- Epitope
- Phospho-Progesterone Receptor (Ser400) Antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Serine 400.
- Gene Name
- PGR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human Progesterone Receptor around the phosphorylation site of Serine 400.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS