Western blot analysis of extracts of BT-474 cells, using PGR antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

PR / Progesterone Receptor Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human, Rat
Format:	Liquid

See all Human PR / Progesterone Receptor antibodies â†’

$281.00each

Catalog Number: LS-C746725-20

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Quantity

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Overview

Description: Progesterone Receptor antibody LS-C746725 is an unconjugated rabbit polyclonal antibody to Progesterone Receptor (PR) from human. It is reactive with human and rat. Validated for IHC and WB.
Application: IHC, WB
Reactivity: Hu, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: NR3C3 | PGR | PR | Progesterone receptor
Recommended Dilution: IHC: 1:50 - 1:100
Recommended Dilution: WB: 1:200 - 1:500
UniProt Number: P06401
NCBI GenBank: AAS00096.1 | NM_000926
SwissProt: P06401

Target

Target: Human PR / Progesterone Receptor
Antigen Species: Human
Gene Name: PGR

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PGR (NP_000917.3). MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDPPAAPATQRVLSPLMSRSGCKVGDSSGTAAAHKVLPRGLSPARQLLLPASESPHWSGAPVKPSPQAAAVEVEEEDGSESEESAGPLLKGKPRALGGAAAG
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume: 20 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: PBS

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