Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with l-Ptase.
PPP1R1B / DARPP-32 Antibody (phospho-Thr34)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Chicken, Dog, Human, Mouse, Rat |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C25-100
Catalog Number: LS-C25
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- Description
- DARPP-32 antibody LS-C25 is an unconjugated rabbit polyclonal antibody to DARPP-32 (PPP1R1B) (pThr34) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Bov, Can, Ck, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DARPP-32 | PPP1R1B | DARPP32 | Phospho-Thr34 DARPP-32
- Recommended Dilution: IHC
- 1:25
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9UD71
- NCBI GenBank
- NM_032192 | NP_115568.2
- SwissProt
- Q9UD71
Target
- Target
- Rat PPP1R1B / DARPP-32
- Antigen Species
- Rat
- Epitope
- Specific for the ~32k DARPP-32 protein phosphorylated at Thr34. Immunolabeling is blocked by a,-phosphatase treatment
- Gene Name
- PPP1R1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phosphoThr3 of rat DARPP-32.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl