Immunofluorescence staining of PPP1R1B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPP1R1B polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
PPP1R1B / DARPP-32 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Cynomolgus Monkey, Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817027-100
Catalog Number: LS-C817027
For bulk orders or custom formulations:
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- Description
- DARPP-32 antibody LS-C817027 is an unconjugated rabbit polyclonal antibody to DARPP-32 (PPP1R1B) from human. It is reactive with human and crab-eating macaque. Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Cyno, Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DARPP-32 | PPP1R1B | DARPP32
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:100 - 1:400
- UniProt Number
- Q9UD71
- NCBI GenBank
- NM_032192 | NP_115568.2
- SwissProt
- Q9UD71
Target
- Target
- Human PPP1R1B / DARPP-32
- Antigen Species
- Human
- Epitope
- Human PPP1R1B
- Gene Name
- PPP1R1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human PPP1R1B fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS