The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: K562 cell lysate Lane 6.: MCF7 cell lysate Lane 7.: LnCap cell lysate
PPIE / Cyclophilin E Antibody (clone AT17E8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755547-50
Catalog Number: LS-C755547
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Cyclophilin E antibody LS-C755547 is an unconjugated mouse monoclonal antibody to human Cyclophilin E (PPIE). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Cyclophilin-33 | CYP33 | Cyclophilin E | CYP-33 | PPIE | PPIase E | Rotamase E
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9UNP9
- NCBI GenBank
- NM_006112 | NP_006103.1
- SwissProt
- Q9UNP9
Target
- Target
- Human PPIE / Cyclophilin E
- Antigen Species
- Human
- Gene Name
- PPIE
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT17E8
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human PPIE (1-301aa) purified from E. coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Recommended Storage Buffer
- PBS