HeLa lysate run on 4-12% Bis-Tris 2D gel in 1xMOPS running buffer. Transfer to 0.45u, nitrocellulose. Membrane probed with 2G9 (anti-PP2A B55a). Anti-mouse IgG (whole molecule)-AP conjugate (1 in 2000). Detection with BCIP/NBT substrate.
PP2Ac / PPP2CA Antibody (clone 2G9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$492.00each
Catalog Number: LS-C827927-0.1
Catalog Number: LS-C827927
For bulk orders or custom formulations:
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- Description
- PPP2CA antibody LS-C827927 is an unconjugated mouse monoclonal antibody to PPP2CA (PP2Ac) from rat. It is reactive with human, mouse and rat. Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Chicken, Pig, Rabbit, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- PP2Ac | PP2Calpha | ppp2A1 | Protein phosphatase 2A alpha | Replication protein C | PP2CA | PPP2CA | RP-C | PP2A-alpha
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P67775
- NCBI GenBank
- NM_002715 | NP_002706.1
- SwissProt
- P67775
Target
- Target
- Rat PP2Ac / PPP2CA
- Antigen Species
- Rat
- Epitope
- Recognizes very well isoforms alpha and rat delta, weakly the human beta isoform, very weakly the rabbit gamma isoform
- Gene Name
- PPP2CA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 2G9
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Peptide from rat PP2A isoform alpha
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS