LSBio
Polyclonal Sheep Anti-Human IgG Secondary Antibody (HRP, IHC, WB)

ELISA results of purified Sheep Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. substrate.

Polyclonal Sheep Anti-Human IgG Secondary Antibody (HRP, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Sheep Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
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$349.00each
Catalog Number: LS-C60637-2

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Overview

Description
Human IgG antibody LS-C60637 is an HRP-conjugated sheep polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:150000 - 1:250000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:10000

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Human IgG and Human Serum.

Antibody

Host Species
Sheep
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.