Western Blot of Anti-Rabbit IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins) Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane.

Polyclonal Rat Anti-Rabbit IgG Secondary Antibody (AP, IHC, WB)

Secondary AntibodyLSBio Guarantee

Antibody:	Rat Polyclonal
Applications:	Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Rabbit
Format:	Liquid

$448.00each

Catalog Number: LS-C60922-1

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Rabbit IgG antibody LS-C60922 is an AP-conjugated rat polyclonal antibody. Validated for ELISA, IHC and WB.
Application: DB, ELISA, IHC, WB
Reactivity: Rb
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Secondary Antibodies
Recommended Dilution: ELISA: 1:1500 - 1:6000
Recommended Dilution: IHC: 1:200 - 1:1000
Recommended Dilution: WB: 1:500 - 1:2000

Target

Target: Rabbit IgG
Target Heavy Chain: IgG
Antigen Species: Rb
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against the sources listed above.

Antibody

Host Species: Rt
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Rabbit IgG whole molecule
Immunogen Type: Native

Format

Conjugate/Label: AP
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: AP