Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Mre11 (S. cerevisiae) Antibody - Chromatin Immunoprecipitation (ChIP). Chromatin Immunoprecipitation (ChIP) using Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. immune complexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorimeter. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Polyclonal Rabbit anti-Saccharomyces cerevisiae MRE11A / MRE11 Antibody (WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | S. cerevisiae |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18848-100
Catalog Number: LS-C18848
For bulk orders or custom formulations:
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- Description
- MRE11 antibody LS-C18848 is an unconjugated rabbit polyclonal antibody to saccharomyces cerevisiae MRE11 (MRE11A). Validated for ChrIP, ELISA and WB.
- Application
- ChIP, ELISA, IHC, WB
- Reactivity
- S.cer
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AT-like disease | Endo/exonuclease Mre11 | HNGS1 | MRE11 | MRE11 homolog 1 | MRE11 homolog A | MRE11B | ATLD | MRE11A
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P49959
- NCBI GenBank
- NM_005591 | NP_005582.1
- SwissProt
- P49959
Target
- Target
- Saccharomyces cerevisiae MRE11A / MRE11
- Antigen Species
- S. cerevisiae
- Epitope
- A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
- Gene Name
- MRE11
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.17 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
