LSBio
Polyclonal Rabbit anti-Rat MAPK3 / ERK1 Antibody (phospho-Thr202/Tyr204, IHC, WB)

Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase)

Polyclonal Rabbit anti-Rat MAPK3 / ERK1 Antibody (phospho-Thr202/Tyr204, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Bovine, Chicken, Dog, Human, Mouse, Rat, Xenopus
Format:Liquid
See all Rat MAPK3 / ERK1 antibodies →
$479.00each
Catalog Number: LS-C26-100

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Overview

Description
ERK1 antibody LS-C26 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Application
IHC, WB
Reactivity
Bov, Can, Ck, Hu, Ms, Rt, Xen
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | MAPK3 | PRKM3 | Phospho-Thr202/Tyr204 ERK/MAPK
Recommended Dilution: IHC
1:25
Recommended Dilution: WB
1:1000
UniProt Number
P27361
SwissProt
P27361

Target

Target
Rat MAPK3 / ERK1
Antigen Species
Rat
Epitope
Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by X phosphatase.
Gene Name
MAPK3

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A phosphopeptide corresponding to amino acid residues surrounding the phosphoThr202 and Tyr204 of rat ERK/MAPK.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
Volume
100 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -20°C.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

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