LSBio
Polyclonal Rabbit anti-Rat CRP / C-Reactive Protein Antibody (IHC, WB)

IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody. C Reactive Protein was detected in paraffin-embedded section of rat cerebellum tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-C Reactive Protein Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Polyclonal Rabbit anti-Rat CRP / C-Reactive Protein Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Rat
Format:Lyophilized
See all Rat CRP / C-Reactive Protein antibodies →
$480.00each
Catalog Number: LS-C756386-100

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Overview

Description
C-Reactive Protein antibody LS-C756386 is an unconjugated rabbit polyclonal antibody to rat C-Reactive Protein (CRP). Validated for ELISA, IHC and WB.
Application
ELISA, IHC-P, WB
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
C-reactive protein | CRP | Pentraxin 1 | PTX1
Recommended Dilution: ELISA
0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P
0.5 - 1 µg/ml
Recommended Dilution: WB
0.1 - 0.5 µg/ml
UniProt Number
P02741
SwissProt
P02741

Target

Target
Rat CRP / C-Reactive Protein
Antigen Species
Rat
Epitope
No cross reactivity with other proteins.
Gene Name
CRP

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived rat C Reactive Protein recombinant protein (Position: H20-S230).
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

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