Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Mouse IL-6 Antibody - Western Blot. Anti-mouse IL-6 antibody in western blot shows detection of recombinant mouse IL-6 raised in E. coli. Recombinant truncated protein (0.1 ug, 21.7 kD) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Polyclonal Rabbit anti-Mouse IL6 / Interleukin 6 Antibody (IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C154128-100
Catalog Number: LS-C154128
For bulk orders or custom formulations:
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- Description
- Interleukin 6 antibody LS-C154128 is an unconjugated rabbit polyclonal antibody to mouse Interleukin 6 (IL6). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | IL6 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P05231
- NCBI GenBank
- NM_000600 | NP_000591.1
- SwissProt
- P05231
Target
- Target
- Mouse IL6 / Interleukin 6
- Antigen Species
- Mouse
- Epitope
- This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-6 from other sources has not been determined.
- Gene Name
- IL6
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
