Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western Blot of Rabbit anti-Mouse IL-1 Antibody Biotin Conjugated. Lane 1: Mouse IL-1 Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1 Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Polyclonal Rabbit anti-Mouse IL-1B / IL-1 Beta Antibody (Biotin, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C154143-100
Catalog Number: LS-C154143
For bulk orders or custom formulations:
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- Description
- IL-1 Beta antibody LS-C154143 is a biotin-conjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) from mouse. It is reactive with mouse and rat. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB.
- Application
- ELISA, FC, IF, IHC, IP, Neut, RIA, WB
- Reactivity
- Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- IL-1B | IL1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P01584
- NCBI GenBank
- NM_000576 | NP_000567.1
- SwissProt
- P01584
Target
- Target
- Mouse IL-1B / IL-1 Beta
- Antigen Species
- Mouse
- Epitope
- This antibody was heated to 56° C for 30 minutes. This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW mouse IL-1β and is useful in determining its presence in various assays. The antibody does not recognize human IL-1β or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1β. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1β containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1β precursor molecule. Denatured 31,000 precursor IL-1β will be recognized by this antibody.
- Gene Name
- IL1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was prepared by repeated immunizations with recombinant mouse IL-1β produced in E.coli. The MW of recombinant mouse IL-1β was 17 kDa.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
