LSBio
Polyclonal Rabbit Anti-Mouse IgG Secondary Antibody (RPE, IF)

Polyclonal Rabbit Anti-Mouse IgG Secondary Antibody (RPE, IF)

Secondary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunofluorescence
Reactivity:Mouse
Format:Lyophilized
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$421.00each
Catalog Number: LS-C209473-1

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Overview

Description
Mouse IgG antibody LS-C209473 is an RPE-conjugated rabbit polyclonal antibody. Validated for Flow and IF.
Application
FC, IF
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:100 - 1:250
Recommended Dilution: IF/ICC
1:100 - 1:250

Target

Target
Mouse IgG
Antigen Species
Mouse
Epitope
This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Phycoerythrin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.5 mg/mL
Volume
1 Milliliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.