LSBio
Polyclonal Rabbit anti-Mouse HIF1A / HIF1 Alpha Antibody (Biotin, aa530-825, IHC, WB)

Polyclonal Rabbit anti-Mouse HIF1A / HIF1 Alpha Antibody (Biotin, aa530-825, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Non-Human Primate, Rat
Format:Liquid
See all Mouse HIF1A / HIF1 Alpha antibodies →
$853.00each
Catalog Number: LS-C121234-100

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Overview

Description
HIF1 Alpha antibody LS-C121234 is a biotin-conjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Recommended Dilution: IHC-P
5 - 10 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q16665
SwissProt
Q16665

Target

Target
Mouse HIF1A / HIF1 Alpha
Antigen Species
Mouse
Epitope
Recognizes mouse HIF-1 alpha. Species cross-reactivity: rat, human, primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples.
Gene Name
HIF1A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
PBS, No preservatives added
Volume
100 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
PBS

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