LSBio
Polyclonal Rabbit anti-Human ZAP70 Antibody (phospho-Tyr319)

Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 ug/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), anti-phospho Zap70 (Y319) in H2O2 treated (blue).

Polyclonal Rabbit anti-Human ZAP70 Antibody (phospho-Tyr319)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunoprecipitation
Reactivity:Human
Format:Liquid
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$301.00each
Catalog Number: LS-C288768-10

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Overview

Description
ZAP70 antibody LS-C288768 is an unconjugated rabbit polyclonal antibody to human ZAP70 (pTyr319). Validated for Flow and IP.
Application
FC, IP
Reactivity
Hu
Predicted Reactivity
Mouse
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | ZAP70 | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
UniProt Number
P43403
NCBI GenBank
NM_207519
SwissProt
P43403

Target

Target
Human ZAP70
Antigen Species
Human
Epitope
Region including tyrosine 319 (when it is phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kD using the numbering given in entry NP_001070.2 (GeneID 7535).
Gene Name
ZAP70

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
A portion of GeneID: 7535 corresponding to amino acids surrounding tyrosine 319 of UniprotID: P43403

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 2-8°C for up to one year.
Recommended Storage Buffer
Tris-citrate

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