LSBio
Polyclonal Rabbit anti-Human Vimentin Antibody (aa435-466, IHC, WB)

Vimentin antibody Western blot. All lanes: Anti Vimentin at 0.5 ug/ml. Lane 1: HT1080 Whole Cell Lysate at 40 ug. Lane 2: NIH Whole Cell Lysate at 40 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Lane 4: HUT Whole Cell Lysate at 40 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Lane 7: Human Placenta Tissue Lysate at 50 ug. Lane 8: Rat Testis Tissue Lysate at 50 ug. Lane 9: Mouse Testis Tissue Lysate at 50 ug. Predicted band size: 54 kD. Observed band size: 54 kD.

Polyclonal Rabbit anti-Human Vimentin Antibody (aa435-466, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Chicken, Guinea Pig, Hamster, Human, Mouse, Non-Human Primate, Rat, Sheep
Format:Lyophilized
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$480.00each
Catalog Number: LS-C357556-100

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Overview

Description
Vimentin antibody LS-C357556 is an unconjugated rabbit polyclonal antibody to Vimentin (aa435-466) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Bov, Ck, GP, Ham, Hu, Ms, NHP, Rt, Sh
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
VIM | Vimentin | vimenin
UniProt Number
P08670
NCBI GenBank
NM_003380, NP_003371.2
SwissProt
P08670

Target

Target
Human Vimentin
Antigen Species
Hu
Epitope
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .
Gene Name
VIM

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Vimentin (435-466 aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Recommended Storage Buffer
NaCl

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