Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Peroxidase - Western Blot. Western Blot showing detection of Recombinant Human VEGF-165. 50 ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20?, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugated - 209-403-B99 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 19 kD molecular weight position expected for rH-VEGF-165. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Polyclonal Rabbit anti-Human VEGFA / VEGF Antibody (HRP, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C154110-100
Catalog Number: LS-C154110
For bulk orders or custom formulations:
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- Description
- VEGF antibody LS-C154110 is an HRP-conjugated rabbit polyclonal antibody to human VEGF (VEGFA). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- VPF | Vascular permeability factor | VEGF | VEGF-A | MVCD1 | VEGFA
- Recommended Dilution: IHC
- 1:500 - 1:2500
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P15692
- NCBI GenBank
- NM_003376 | NP_003367.4
- SwissProt
- P15692
Target
- Target
- Human VEGFA / VEGF
- Antigen Species
- Human
- Epitope
- This antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
- Gene Name
- VEGFA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
