Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western Blot of rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin. Other band(s): Not identified
Polyclonal Rabbit anti-Human VDAC1 / PORIN Antibody (IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Goat, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745298-25
Catalog Number: LS-C745298
For bulk orders or custom formulations:
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- Description
- PORIN antibody LS-C745298 is an unconjugated rabbit polyclonal antibody to PORIN (VDAC1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Bov, Gt, Hu, Ms, Por, Rt
- Predicted Reactivity
- Bird / Avian (Other), Fish (Other), Mammal (Other), Reptile
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HVDAC1 | Plasmalemmal porin | Porin 31HM | PORIN | PORIN-31-HL | Vdac5 | VDAC | VDAC1 | Mitochondrial porin | Porin 31HL | VDAC-1
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P21796
- NCBI GenBank
- NM_003374 | NP_003365.1
- SwissProt
- P21796
Target
- Target
- Human VDAC1 / PORIN
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
- Gene Name
- VDAC1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
