LSBio
Polyclonal Rabbit anti-Human UBA52 Antibody (aa1-50, IHC, WB)

Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 ug. Antibody: Affinity purified rabbit anti-Ubiquitin antibody used at 0.4 ug/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).

Polyclonal Rabbit anti-Human UBA52 Antibody (aa1-50, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Bovine, Human, Mouse
Format:Liquid
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$301.00each
Catalog Number: LS-C287223-10

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Overview

Description
UBA52 antibody LS-C287223 is an unconjugated rabbit polyclonal antibody to UBA52 (aa1-50) from human. It is reactive with human, mouse and bovine. Validated for IHC and WB.
Application
IHC, WB
Reactivity
Bov, Hu, Ms
Predicted Reactivity
Cat, Chicken, Dog, Fruit Fly, Guinea Pig, Horse, Non-Human Primate, Pig, Rabbit, Rat, Sheep, Xenopus
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
60S ribosomal protein L40 | HUBCEP52 | L40 | RPL40 | UBA52 | Ubiquitin-CEP52 | UBCEP2 | CEP52
Recommended Dilution: IHC
1:500 - 1:2000
Recommended Dilution: WB
1:1000 - 1:10000
UniProt Number
P62987
NCBI GenBank
NM_003333 | NP_003324.1
SwissProt
P62987

Target

Target
Human UBA52
Antigen Species
Human
Epitope
Region between residues 1 and 50 of human Ubiquitin using the numbering given in entry P62988.(GeneID 7311).
Gene Name
UBA52

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
A portion of GeneID: 7311 corresponding to amino acids between 1 and 50 of UniprotID: P62987
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 2-8°C for up to one year.
Recommended Storage Buffer
Tris-citrate

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